Actinobacillus actinomycetemcomitans(A. actinomycetemcomitans), a nonmotile, gram negative facultative anaerobic coccobacillus, is supposed to be an important etiological agent in localized juvenile periodontitis and associated with adult type periodontitis. Lipopolysaccharide (LPS) is one of the various virulent factors of A. actinomycetemcomitans and can directly or indirectly cause destruction of periodontal tissue. The purpose of this study was to produce and characterize the monoclonal antibody to LPS of A, actinomycetemcomitans.
The results were as follows;
1. The LPS yield of A. actinomycetemcomitans was 0.7% based on dry cell weight.
2. The antibody titers of immuninzed serum with 5.2 x 108 bacteria were 1:64,000 to whole cells and 1:8,000 to LPS.
3. From three times of cloning the antibody producing hybridomas using limitation dilution method, ten
monoclones producing monoclonal antibody to LPS of A. actinomycetemcomitans were obtained.
4. Ten monoclonal antibodies to LPS of A, actinomycetemcomitans were not found to cross-react with LPS
of Escherichia coil (E.. cob).
These results indicate that the monoclonal antibody to LPS of A. actinomycetemcomitans was specific for A. actinomycetemcomitans
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